Ownership Submission
FORM 3
 UNITED STATES SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

INITIAL STATEMENT OF BENEFICIAL OWNERSHIP OF SECURITIES

Filed pursuant to Section 16(a) of the Securities Exchange Act of 1934, Section 17(a) of the Public Utility Holding Company Act of 1935 or Section 30(h) of the Investment Company Act of 1940
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(Print or Type Responses)
1. Name and Address of Reporting Person *
  Costolo Richard
 2. Date of Event Requiring Statement (Month/Day/Year)
11/06/2013		 3. Issuer Name and Ticker or Trading Symbol
TWITTER, INC. [TWTR]
(Last)
(First)
(Middle)
C/O TWITTER, INC., 1355 MARKET STREET, SUITE 900		 4. Relationship of Reporting Person(s) to Issuer
(Check all applicable)
__X__ Director _____ 10% Owner
__X__ Officer (give title below) _____ Other (specify below)
Chief Executive Officer
 5. If Amendment, Date Original Filed(Month/Day/Year)
(Street)

SAN FRANCISCO, CA 94103		 6. Individual or Joint/Group Filing(Check Applicable Line)
_X_ Form filed by One Reporting Person
___ Form filed by More than One Reporting Person
(City)
(State)
(Zip)
Table I - Non-Derivative Securities Beneficially Owned
1.Title of Security
(Instr. 4)		 2. Amount of Securities Beneficially Owned
(Instr. 4)		 3. Ownership Form: Direct (D) or Indirect (I)
(Instr. 5)		 4. Nature of Indirect Beneficial Ownership
(Instr. 5)
Common Stock 566,920
I
See footnote (1)

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly. SEC 1473 (7-02)
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Table II - Derivative Securities Beneficially Owned (e.g., puts, calls, warrants, options, convertible securities)
1. Title of Derivative Security
(Instr. 4)		 2. Date Exercisable and Expiration Date
(Month/Day/Year)		 3. Title and Amount of Securities Underlying Derivative Security
(Instr. 4)		 4. Conversion or Exercise Price of Derivative Security 5. Ownership Form of Derivative Security: Direct (D) or Indirect (I)
(Instr. 5)		 6. Nature of Indirect Beneficial Ownership
(Instr. 5)
Date Exercisable Expiration Date Title Amount or Number of Shares
Employee Stock Option (right to buy)   (2) 09/03/2019 Common Stock 3,752,606 $ 0.4283 D  
Employee Stock Option (right to buy)   (3) 11/22/2020 Common Stock 4,110,284 $ 1.83 D  
Employee Stock Option (right to buy)   (4) 04/11/2022 Common Stock 388,440 $ 14.42 D  
Employee Stock Option (right to buy)   (2) 09/03/2019 Common Stock 273,000 $ 0.4283 I See footnote (5)

Reporting Owners

Reporting Owner Name / Address Relationships
Director 10% Owner Officer Other
Costolo Richard
C/O TWITTER, INC.
1355 MARKET STREET, SUITE 900
SAN FRANCISCO, CA 94103		   X     Chief Executive Officer  

Signatures

/s/ Sean Edgett, attorney-in-fact 11/06/2013
**Signature of Reporting Person Date

Explanation of Responses:

* If the form is filed by more than one reporting person, see Instruction 5(b)(v).
** Intentional misstatements or omissions of facts constitute Federal Criminal Violations. See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).
(1) The shares are held of record by the Richard Costolo 2001 Living Trust dated February 8, 2001, for which the Reporting Person serves as trustee, and the Lorin Costolo 2001 Living Trust dated February 8, 2001, for which the Reporting Person's spouse serves as trustee.
(2) The option is fully vested and immediately exercisable.
(3) One-fourth of the shares subject to the option vested on November 22, 2011 and 1/48th of the shares vest monthly thereafter.
(4) 6.25% of the shares subject to the option vest on each of July 1, 2014, October 1, 2014, January 1, 2015 and April 1, 2015, and 18.75% of the shares subject to the option vest on each of of July 1, 2015, October 1, 2015, January 1, 2016 and April 1, 2016.
(5) The option is held of record by the Northern Trust Company, as Trustee of the Lorin Costolo 2012 Gift Trust. Ms. Costolo is the spouse of the Reporting Person.
 
Remarks:
Exhibit 24 - Power of Attorney

The Reporting Person holds restricted stock units awarded prior to February 1, 2013 (the "Pre-2013 RSUs"), the vesting of which is subject to the satisfaction of both a service condition and a performance condition. Accordingly, the Pre-2013 RSUs are not yet reportable for purposes of Section 16 and are not included in this Form 3. The performance condition will be satisfied on the earlier of (i) the date that is the earlier of (x) six months after the effective date of the Issuer's initial public offering or (y) March 8th of the calendar year following the effective date of the Issuer's initial public offering (which the Issuer may elect to accelerate to February 15th); and (ii) the date of a change in control.

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